Ra's al Ghul had a child born during his travels in Russia in the 18th Century named Nyssa. Enamoured by the romantic stories that her mother would tell her about Ra's as a child, Nyssa set out to find Ra's and eventually located him at his headquarters in North Africa.
Impressed by her beauty, her warrior skills, and the fact that she was able to locate him, Ra's promoted her to a position similar to that held by her half-sister, Talia. As a right hand associate, she accompanied Ra's during his adventures. Ra's was so impressed with her abilities, he even allowed Nyssa to use his Lazarus Pits. Like her sister Talia, Nyssa eventually became disenchanted with Ra's genocidal plans to "cleanse the Earth," and disassociated herself from her father sometime in the early 19th century. Ra's reluctantly approved this, believing that she would return to him and that she and/or her children would become his future heirs. To his disappointment, Nyssa refused to give herself or her family to him, causing him officially to disown her permanently. He did however allow her to keep a Lazarus Pit for herself and, much to his surprise, she found a way to reuse it, allowing her to survive until modern times.
During World War II, Nyssa and her family were sent to a concentration camp, where Nyssa's entire family died and she was rendered infertile by gruesome Mengele-sque experiments.